A DNA cassette containing a trimerized SV40 polyadenylation signal which efficiently blocks spurious plasmid-initiated transcription.
IH Maxwell, GS Harrison, WM Wood, F Maxwell - Biotechniques, 1989 - europepmc.org
IH Maxwell, GS Harrison, WM Wood, F Maxwell
Biotechniques, 1989•europepmc.orgA head-to-tail trimer of the SV40 Bcl I-Bam H1 DNA fragment, specifying polyadenylation of
RNA transcripts, was cloned as a cassette flanked by multiple restriction sites. Insertion of
the trimer into several expression vectors efficiently prevented spurious expression of
reporter genes resulting from transcriptional initiation in prokaryotic plasmid sequences in
transfected mammalian cells.
RNA transcripts, was cloned as a cassette flanked by multiple restriction sites. Insertion of
the trimer into several expression vectors efficiently prevented spurious expression of
reporter genes resulting from transcriptional initiation in prokaryotic plasmid sequences in
transfected mammalian cells.
A head-to-tail trimer of the SV40 Bcl I-Bam H1 DNA fragment, specifying polyadenylation of RNA transcripts, was cloned as a cassette flanked by multiple restriction sites. Insertion of the trimer into several expression vectors efficiently prevented spurious expression of reporter genes resulting from transcriptional initiation in prokaryotic plasmid sequences in transfected mammalian cells.
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