[HTML][HTML] Validation of lucigenin (bis-N-methylacridinium) as a chemilumigenic probe for detecting superoxide anion radical production by enzymatic and cellular …
Y Li, H Zhu, P Kuppusamy, V Roubaud… - Journal of Biological …, 1998 - ASBMB
Lucigenin is most noted for its wide use as a chemiluminescent detector of superoxide anion
radical (O· ̄ 2) production by biological systems. However, its validity as a O· ̄ 2-detecting
probe has recently been questioned in view of its ability to undergo redox cycling in several
in vitroenzymatic systems, which produce little or no O· ̄ 2. Whether and to what extent
lucigenin redox cycling occurs in systems that produce significant amounts of O· ̄ 2 has not
been carefully investigated. We examined and correlated three end points, including …
radical (O· ̄ 2) production by biological systems. However, its validity as a O· ̄ 2-detecting
probe has recently been questioned in view of its ability to undergo redox cycling in several
in vitroenzymatic systems, which produce little or no O· ̄ 2. Whether and to what extent
lucigenin redox cycling occurs in systems that produce significant amounts of O· ̄ 2 has not
been carefully investigated. We examined and correlated three end points, including …