[HTML][HTML] Intracellular generation of sphingosine 1-phosphate in human lung endothelial cells: role of lipid phosphate phosphatase-1 and sphingosine kinase 1
Sphingosine 1-phosphate (S1P) regulates diverse cellular functions through extracellular
ligation to S1P receptors, and it also functions as an intracellular second messenger. Human
pulmonary artery endothelial cells (HPAECs) effectively utilized exogenous S1P to generate
intracellular S1P. We, therefore, examined the role of lipid phosphate phosphatase (LPP)-1
and sphingosine kinase1 (SphK1) in converting exogenous S1P to intracellular S1P.
Exposure of 32 P-labeled HPAECs to S1P or sphingosine (Sph) increased the intracellular …
ligation to S1P receptors, and it also functions as an intracellular second messenger. Human
pulmonary artery endothelial cells (HPAECs) effectively utilized exogenous S1P to generate
intracellular S1P. We, therefore, examined the role of lipid phosphate phosphatase (LPP)-1
and sphingosine kinase1 (SphK1) in converting exogenous S1P to intracellular S1P.
Exposure of 32 P-labeled HPAECs to S1P or sphingosine (Sph) increased the intracellular …