A simple method for the gas–liquid chromatographic quantitation of human fecal bile acids
and sterols is described where bile acids are subjected to n-butyl ester derivatization,
without prior isolation from the stool, followed by trimethylsilylation of the sterols and bile
acids. Under these conditions, bile acid derivatives are well resolved from each other and
from the trimethylsilyl ether derivatives of fecal sterols and no overlap occurs. The method
was shown to be highly reproducible and recoveries were similar to those obtained with …