Calcium Phosphate in Osteogenic Cells: A Critique of the Glyoxal Bis (2-Hydroxyanil) and the Dilute Silver Acetate Methods.

HK Kashiwa - Clinical Orthopaedics and Related Research (1976 …, 1970 - journals.lww.com
Most of the histochemical methods for localizing calcium are for insoluble calcium
phosphate or carbonate salts. These methods are described in histochemical textbooks, and
the problems associated with their use have been reviewed by Cameron," and more recently
by McGhee-Russell. This paper will review only the glyoxal bis (2-hydroxy-anil)(GBHA)
method-13, 22 for cellular calcium, and the dilute Ag acetate method? for cellular phosphate
or carbonate. These cytochemical methods were developed pri-marily to permit localization …