Presence of fetal RNA in maternal plasma
LLM Poon, TN Leung, TK Lau, YMD Lo - Clinical chemistry, 2000 - academic.oup.com
LLM Poon, TN Leung, TK Lau, YMD Lo
Clinical chemistry, 2000•academic.oup.comThe discovery of fetal DNA in maternal plasma (1) has opened up a new horizon on prenatal
molecular diagnosis. Many groups have since shown that fetal genetic traits, such as RhD
status and inherited genetic diseases, can be determined from fetal DNA in maternal plasma
(2–5). However, it is not known whether fetal RNA is also present in maternal plasma. Here,
using a two-step reverse transcription (RT)-PCR assay, we demonstrate the presence of
fetal-derived, male-specific mRNA in plasma of pregnant women carrying male fetuses …
molecular diagnosis. Many groups have since shown that fetal genetic traits, such as RhD
status and inherited genetic diseases, can be determined from fetal DNA in maternal plasma
(2–5). However, it is not known whether fetal RNA is also present in maternal plasma. Here,
using a two-step reverse transcription (RT)-PCR assay, we demonstrate the presence of
fetal-derived, male-specific mRNA in plasma of pregnant women carrying male fetuses …
The discovery of fetal DNA in maternal plasma (1) has opened up a new horizon on prenatal molecular diagnosis. Many groups have since shown that fetal genetic traits, such as RhD status and inherited genetic diseases, can be determined from fetal DNA in maternal plasma (2–5). However, it is not known whether fetal RNA is also present in maternal plasma. Here, using a two-step reverse transcription (RT)-PCR assay, we demonstrate the presence of fetal-derived, male-specific mRNA in plasma of pregnant women carrying male fetuses. Pregnant women attending the Prenatal Diagnosis Unit at the Department of Obstetrics and Gynecology, Prince of Wales Hospital, Hong Kong were recruited with informed
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