[HTML][HTML] Mice completely lacking immunoproteasomes show major changes in antigen presentation

EZ Kincaid, JW Che, I York, H Escobar… - Nature …, 2012 - nature.com
EZ Kincaid, JW Che, I York, H Escobar, E Reyes-Vargas, JC Delgado, RM Welsh, ML Karow…
Nature immunology, 2012nature.com
The importance of immunoproteasomes to antigen presentation has been unclear because
animals totally lacking immunoproteasomes had not been available. Having now developed
mice lacking the three immunoproteasome catalytic subunits, we found that the dendritic
cells of these mice had defects in presenting several major histocompatibility complex
(MHC) class I epitopes. During viral infection in vivo, the presentation of a majority of MHC
class I epitopes was markedly reduced in immunoproteasome-deficient animals compared …
Abstract
The importance of immunoproteasomes to antigen presentation has been unclear because animals totally lacking immunoproteasomes had not been available. Having now developed mice lacking the three immunoproteasome catalytic subunits, we found that the dendritic cells of these mice had defects in presenting several major histocompatibility complex (MHC) class I epitopes. During viral infection in vivo, the presentation of a majority of MHC class I epitopes was markedly reduced in immunoproteasome-deficient animals compared with wild-type animals, whereas presentation of MHC class II peptides was unaffected. According to mass spectrometry, the repertoire of MHC class I–presented peptides was ∼50% different from that in wild-type mice, and these differences were sufficient to stimulate robust transplant rejection of wild-type cells in mutant mice. These results indicated that immunoproteasomes were more important in antigen presentation than previously thought.
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