DNA electroporation, isolation and imaging of myofibers

AR Demonbreun, EM McNally - JoVE (Journal of Visualized Experiments), 2015 - jove.com
Mature muscle has a unique structure that is amenable to live cell imaging. Herein, we
describe the experimental protocol for expressing fluorescently labeled proteins in the flexor
digitorum brevis (FDB) muscle. Conditions have been optimized to provide a large number
of high quality myofibers expressing the electroporated plasmid while minimizing muscle
damage. The method employs fluorescent tags on various proteins. Combining this
expression method with high resolution confocal microscopy permits live cell imaging …