Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

S Konermann, MD Brigham, AE Trevino, J Joung… - Nature, 2015 - nature.com
S Konermann, MD Brigham, AE Trevino, J Joung, OO Abudayyeh, C Barcena, PD Hsu
Nature, 2015nature.com
Systematic interrogation of gene function requires the ability to perturb gene expression in a
robust and generalizable manner. Here we describe structure-guided engineering of a
CRISPR-Cas9 complex to mediate efficient transcriptional activation at endogenous
genomic loci. We used these engineered Cas9 activation complexes to investigate single-
guide RNA (sgRNA) targeting rules for effective transcriptional activation, to demonstrate
multiplexed activation of ten genes simultaneously, and to upregulate long intergenic non …
Abstract
Systematic interrogation of gene function requires the ability to perturb gene expression in a robust and generalizable manner. Here we describe structure-guided engineering of a CRISPR-Cas9 complex to mediate efficient transcriptional activation at endogenous genomic loci. We used these engineered Cas9 activation complexes to investigate single-guide RNA (sgRNA) targeting rules for effective transcriptional activation, to demonstrate multiplexed activation of ten genes simultaneously, and to upregulate long intergenic non-coding RNA (lincRNA) transcripts. We also synthesized a library consisting of 70,290 guides targeting all human RefSeq coding isoforms to screen for genes that, upon activation, confer resistance to a BRAF inhibitor. The top hits included genes previously shown to be able to confer resistance, and novel candidates were validated using individual sgRNA and complementary DNA overexpression. A gene expression signature based on the top screening hits correlated with markers of BRAF inhibitor resistance in cell lines and patient-derived samples. These results collectively demonstrate the potential of Cas9-based activators as a powerful genetic perturbation technology.
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