Protective role of testosterone in ischemia-reperfusion-induced acute kidney injury

A Soljancic, AL Ruiz… - American Journal …, 2013 - journals.physiology.org
A Soljancic, AL Ruiz, K Chandrashekar, R Maranon, R Liu, JF Reckelhoff, LA Juncos
American Journal of Physiology-Regulatory, Integrative and …, 2013journals.physiology.org
Men are at greater risk for renal injury and dysfunction after acute ischemia-reperfusion (I/R)
than are women. Studies in animals suggest that the reason for the sex difference in renal
injury and dysfunction after I/R is the protective effect of estrogens in females. However, a
reduction in testosterone in men is thought to play an important role in mediating
cardiovascular and renal disease, in general. In the present study, we tested the hypothesis
that I/R of the kidney reduces serum testosterone, and that contributes to renal dysfunction …
Men are at greater risk for renal injury and dysfunction after acute ischemia-reperfusion (I/R) than are women. Studies in animals suggest that the reason for the sex difference in renal injury and dysfunction after I/R is the protective effect of estrogens in females. However, a reduction in testosterone in men is thought to play an important role in mediating cardiovascular and renal disease, in general. In the present study, we tested the hypothesis that I/R of the kidney reduces serum testosterone, and that contributes to renal dysfunction and injury. Male rats that were subjected to renal ischemia of 40 min followed by reperfusion had a 90% reduction in serum testosterone by 3 h after reperfusion that remained at 24 h. Acute infusion of testosterone 3 h after reperfusion attenuated the increase in plasma creatinine and urinary kidney injury molecule-1 (KIM-1) at 24 h, prevented the reduction in outer medullary blood flow, and attenuated the increase in intrarenal TNF-α and the decrease in intrarenal VEGF at 48 h. Castration of males caused greater increases in plasma creatinine and KIM-1 at 24 h than in intact males with renal I/R, and treatment with anastrozole, an aromatase inhibitor, plus testosterone almost normalized plasma creatinine and KIM-1 in rats with renal I/R. These data show that renal I/R is associated with sustained reductions in testosterone, that testosterone repletion protects the kidney, whereas castration promotes renal dysfunction and injury, and that the testosterone-mediated protection is not conferred by conversion to estradiol.
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