[HTML][HTML] Protein-tyrosine phosphatase 1B complexes with the insulin receptor in vivo and is tyrosine-phosphorylated in the presence of insulin
D Bandyopadhyay, A Kusari, KA Kenner, F Liu… - Journal of Biological …, 1997 - ASBMB
In response to insulin, protein-tyrosine phosphatase 1B (PTPase 1B) dephosphorylates 95-
and 160-180-kDa tyrosine phosphorylated (PY) proteins (Kenner, KA, Anyanwu, E., Olefsky,
JM, and Kusari, J.(1996) J. Biol. Chem. 271, 19810-19816). To characterize these proteins,
lysates from control and insulin-treated cells expressing catalytically inactive PTPase 1B
(CS) were immunoadsorbed and subsequently immunoblotted using various combinations
of phosphotyrosine, PTPase 1B, and insulin receptor (IR) antibodies. Anti-PTPase 1B …
and 160-180-kDa tyrosine phosphorylated (PY) proteins (Kenner, KA, Anyanwu, E., Olefsky,
JM, and Kusari, J.(1996) J. Biol. Chem. 271, 19810-19816). To characterize these proteins,
lysates from control and insulin-treated cells expressing catalytically inactive PTPase 1B
(CS) were immunoadsorbed and subsequently immunoblotted using various combinations
of phosphotyrosine, PTPase 1B, and insulin receptor (IR) antibodies. Anti-PTPase 1B …