[HTML][HTML] Structure-guided engineering of human thymidine kinase 2 as a positron emission tomography reporter gene for enhanced phosphorylation of non-natural …
DO Campbell, SS Yaghoubi, Y Su, JT Lee… - Journal of Biological …, 2012 - ASBMB
Positron emission tomography (PET) reporter gene imaging can be used to non-invasively
monitor cell-based therapies. Therapeutic cells engineered to express a PET reporter gene
(PRG) specifically accumulate a PET reporter probe (PRP) and can be detected by PET
imaging. Expanding the utility of this technology requires the development of new non-
immunogenic PRGs. Here we describe a new PRG-PRP system that employs, as the PRG, a
mutated form of human thymidine kinase 2 (TK2) and 2′-deoxy-2′-18 F-5-methyl-1-β-l …
monitor cell-based therapies. Therapeutic cells engineered to express a PET reporter gene
(PRG) specifically accumulate a PET reporter probe (PRP) and can be detected by PET
imaging. Expanding the utility of this technology requires the development of new non-
immunogenic PRGs. Here we describe a new PRG-PRP system that employs, as the PRG, a
mutated form of human thymidine kinase 2 (TK2) and 2′-deoxy-2′-18 F-5-methyl-1-β-l …