[HTML][HTML] DNA-mediated folding and assembly of MyoD-E47 heterodimers
H Wendt, RM Thomas, T Ellenberger - Journal of Biological Chemistry, 1998 - ASBMB
Basic region helix-loop-helix (bHLH) transcription factors regulate key steps in early
development by binding to regulatory DNA sites as heterodimers consisting of a tissue-
specific factor and a widely expressed factor. We have examined the folding, dimerization,
and DNA binding properties of the muscle-specific bHLH protein MyoD and its partner E47,
to understand why these proteins preferentially associate in heterodimeric complexes with
DNA. In the absence of DNA, the E47 bHLH domain forms a very stable homodimer …
development by binding to regulatory DNA sites as heterodimers consisting of a tissue-
specific factor and a widely expressed factor. We have examined the folding, dimerization,
and DNA binding properties of the muscle-specific bHLH protein MyoD and its partner E47,
to understand why these proteins preferentially associate in heterodimeric complexes with
DNA. In the absence of DNA, the E47 bHLH domain forms a very stable homodimer …