Energy-dependent calcium sequestration activity in rat liver microsomes.
L Moore, T Chen, HR Knapp, EJ Landon - Journal of Biological Chemistry, 1975 - ASBMB
MgATP-dependent calcium sequestering activity of rat liver microsomes has been
characterized. This activity is linear over a 90-min period and specifically requires MgATP.
Substitution of CTP, UTP, GTP, or ADP will not support calcium uptake. Oxalate, which
serves as a trapping agent in calcium uptake of skeletal muscle microsomes, is required to
maintain net accumulation of calcium. The reaction is temperature-dependent and has an
apparent Vmax of 11.2 nmol/mg of protein/min. The apparent Km for calcium is 23.2 muM …
characterized. This activity is linear over a 90-min period and specifically requires MgATP.
Substitution of CTP, UTP, GTP, or ADP will not support calcium uptake. Oxalate, which
serves as a trapping agent in calcium uptake of skeletal muscle microsomes, is required to
maintain net accumulation of calcium. The reaction is temperature-dependent and has an
apparent Vmax of 11.2 nmol/mg of protein/min. The apparent Km for calcium is 23.2 muM …