[HTML][HTML] Insulin receptor substrate proteins create a link between the tyrosine phosphorylation cascade and the Ca2+-ATPases in muscle and heart
P Algenstaedt, DA Antonetti, MB Yaffe… - Journal of Biological …, 1997 - ASBMB
Following phosphorylation by the insulin receptor kinase, the insulin receptor substrates
(IRS)-1 and IRS-2 bind to and activate several Src homology 2 (SH2) domain proteins. To
identify novel proteins that interact with IRS proteins in muscle, a human skeletal muscle
cDNA expression library was created in the λEXlox system and probed with baculovirus-
produced and tyrosine-phosphorylated human IRS-1. One clone of the 10 clones which was
positive through three rounds of screening represented the C terminus of the human …
(IRS)-1 and IRS-2 bind to and activate several Src homology 2 (SH2) domain proteins. To
identify novel proteins that interact with IRS proteins in muscle, a human skeletal muscle
cDNA expression library was created in the λEXlox system and probed with baculovirus-
produced and tyrosine-phosphorylated human IRS-1. One clone of the 10 clones which was
positive through three rounds of screening represented the C terminus of the human …