[HTML][HTML] Critical role of ADP interaction with P2Y12 receptor in the maintenance of αIIbβ3 activation: association with Rap1B activation
T Kamae, M Shiraga, H Kashiwagi, H Kato… - Journal of Thrombosis …, 2006 - Elsevier
Summary Objective: Platelet integrin α IIb β 3 plays a crucial role in platelet aggregation, and
the affinity of α IIb β 3 for fibrinogen is dynamically regulated. Employing modified ligand‐
binding assays, we analyzed the mechanism by which α IIb β 3 maintains its high‐affinity
state. Methods and results: Washed platelets adjusted to 50× 10 3 μL− 1 were stimulated
with 0.2 U mL− 1 thrombin or 5 μ m U46619 under static conditions. After the completion of α
IIb β 3 activation and granule secretion, different kinds of antagonists were added to the …
the affinity of α IIb β 3 for fibrinogen is dynamically regulated. Employing modified ligand‐
binding assays, we analyzed the mechanism by which α IIb β 3 maintains its high‐affinity
state. Methods and results: Washed platelets adjusted to 50× 10 3 μL− 1 were stimulated
with 0.2 U mL− 1 thrombin or 5 μ m U46619 under static conditions. After the completion of α
IIb β 3 activation and granule secretion, different kinds of antagonists were added to the …
