Human immunodeficiency virus type 1 vectors efficiently transduce human hematopoietic stem cells
RE Sutton, HTM Wu, R Rigg, E Böhnlein… - Journal of …, 1998 - Am Soc Microbiol
Lentiviruses are potentially advantageous compared to oncoretroviruses as gene transfer
agents because they can infect nondividing cells. We demonstrate here that human
immunodeficiency virus type 1 (HIV-1)-based vectors were highly efficient in transducing
purified human hematopoietic stem cells. Transduction rates, measured by marker gene
expression or by PCR of the integrated provirus, exceeded 50%, and transduction appeared
to be independent of mitosis. Derivatives of HIV-1 were constructed to optimize the vector …
agents because they can infect nondividing cells. We demonstrate here that human
immunodeficiency virus type 1 (HIV-1)-based vectors were highly efficient in transducing
purified human hematopoietic stem cells. Transduction rates, measured by marker gene
expression or by PCR of the integrated provirus, exceeded 50%, and transduction appeared
to be independent of mitosis. Derivatives of HIV-1 were constructed to optimize the vector …
Abstract
Lentiviruses are potentially advantageous compared to oncoretroviruses as gene transfer agents because they can infect nondividing cells. We demonstrate here that human immunodeficiency virus type 1 (HIV-1)-based vectors were highly efficient in transducing purified human hematopoietic stem cells. Transduction rates, measured by marker gene expression or by PCR of the integrated provirus, exceeded 50%, and transduction appeared to be independent of mitosis. Derivatives of HIV-1 were constructed to optimize the vector, and a deletion of most of Vif and Vpr was required to ensure the long-term persistence of transduced cells with relatively stable expression of the marker gene product. These results extend the utility of this lentivirus vector system.
American Society for Microbiology