Capturing genes encoding membrane and secreted proteins important for mouse development.
WC Skarnes, JE Moss, SM Hurtley… - Proceedings of the …, 1995 - National Acad Sciences
WC Skarnes, JE Moss, SM Hurtley, RS Beddington
Proceedings of the National Academy of Sciences, 1995•National Acad SciencesA strategy based on the gene trap was developed to prescreen mouse embryonic stem cells
for insertional mutations in genes encoding secreted and membrane-spanning proteins.
The" secretory trap" relies on capturing the N-terminal signal sequence of an endogenous
gene to generate an active beta-galactosidase fusion protein. Insertions were found in a
cadherin gene, an unc6-related laminin (netrin) gene, the sek receptor tyrosine kinase gene,
and genes encoding two receptor-linked protein-tyrosine phosphatases, LAR and PTP …
for insertional mutations in genes encoding secreted and membrane-spanning proteins.
The" secretory trap" relies on capturing the N-terminal signal sequence of an endogenous
gene to generate an active beta-galactosidase fusion protein. Insertions were found in a
cadherin gene, an unc6-related laminin (netrin) gene, the sek receptor tyrosine kinase gene,
and genes encoding two receptor-linked protein-tyrosine phosphatases, LAR and PTP …
A strategy based on the gene trap was developed to prescreen mouse embryonic stem cells for insertional mutations in genes encoding secreted and membrane-spanning proteins. The "secretory trap" relies on capturing the N-terminal signal sequence of an endogenous gene to generate an active beta-galactosidase fusion protein. Insertions were found in a cadherin gene, an unc6-related laminin (netrin) gene, the sek receptor tyrosine kinase gene, and genes encoding two receptor-linked protein-tyrosine phosphatases, LAR and PTP kappa. Analysis of homozygous mice carrying insertions in LAR and PTP kappa showed that both genes were effectively disrupted, but neither was essential for normal embryonic development.
National Acad Sciences