Abstract A full-length (6.1-kb) human genomic pim-1 gene, together with its immediate 5′-upstream promoter sequence (Ppim) was isolated and sequenced. The human pim-1 gene shares an overall nucleotide (nt) sequence identity of 53% with the previously reported murine pim-1 gene. It consists of six exons and five introns and contains a protein-coding region that is identical in nt sequence to a full-length human pim-1 cDNA. The gene codes for a predicted Pim-1 protein of 313 amino acids (aa) with an M r of 35 690 and a pI of 5.7. The deduced aa sequence of the human Pim-1 has 94% identity with the murine Pim-1 whereas the nt sequences of the two genes are 88% identical. All of the conserved aa residues of the mouse pim-1 gene, which are homologous to known protein kinases are conserved in the predicted human protein. The human Ppim region is very G+ C-rich (69%) and shares greater than 80% identity with the murine Ppim. The Ppim has no TATA-or CAAT-box sequences but does contain a number of nt sequences similar to the putative binding sites of several presumptive transcription factors.