[HTML][HTML] Increased expression of Interleukin-13 but not Interleukin-4 in cystic fibrosis patients
HP Hauber, D Gholami, G Koppermann… - Journal of Cystic …, 2003 - Elsevier
HP Hauber, D Gholami, G Koppermann, HE Heuer, A Meyer, A Pforte
Journal of Cystic Fibrosis, 2003•ElsevierBackground: Many patients with cystic fibrosis (CF) suffer from allergic disease, which can
complicate treatment of CF lung disease. Interleukin (IL)-4 and IL-13 have been shown to be
important mediators in allergic disease. Objective: To investigate the role of IL-4 and IL-13 in
allergic and non-allergic CF patients. Methods: Expression of IL-4 and IL-13 mRNA was
investigated in peripheral blood mononuclear cells (PBM) of seven CF patients with allergy,
of six patients without allergy and of nine healthy subjects as well as in BAL cells of four …
complicate treatment of CF lung disease. Interleukin (IL)-4 and IL-13 have been shown to be
important mediators in allergic disease. Objective: To investigate the role of IL-4 and IL-13 in
allergic and non-allergic CF patients. Methods: Expression of IL-4 and IL-13 mRNA was
investigated in peripheral blood mononuclear cells (PBM) of seven CF patients with allergy,
of six patients without allergy and of nine healthy subjects as well as in BAL cells of four …
Background
Many patients with cystic fibrosis (CF) suffer from allergic disease, which can complicate treatment of CF lung disease. Interleukin (IL)-4 and IL-13 have been shown to be important mediators in allergic disease.
Objective
To investigate the role of IL-4 and IL-13 in allergic and non-allergic CF patients.
Methods
Expression of IL-4 and IL-13 mRNA was investigated in peripheral blood mononuclear cells (PBM) of seven CF patients with allergy, of six patients without allergy and of nine healthy subjects as well as in BAL cells of four patients and of all controls. PBM from six patients were incubated with recombinant human IL-13 or human antiIL-13 antibody without and with LPS stimulation and TNFα levels were measured by ELISA.
Results
IL-13 mRNA expression was increased in allergic and non-allergic patients compared to controls. No significant difference in IL-4 expression could be found between patients and controls. Addition of IL-13 decreased TNFα in PBM culture supernatants.
Conclusion
Our data suggest that IL-13 rather than IL-4 might play an important role in both allergic and non-allergic CF patients. IL-13 might also compromise host defence by decreasing TNFα production.
Elsevier