Fetal membrane distention: II. Differentially expressed genes regulated by acute distention in vitro
E Nemeth, LK Millar, G Bryant-Greenwood - American journal of obstetrics …, 2000 - Elsevier
Objective: This study was undertaken to identify genes with expression up-regulated by
acute distention in the human fetal membranes. Study Design: Fetal membrane explants
were distended reproducibly in a novel device in vitro for 4 hours, and suppression
subtractive hybridization was used to identify the candidate genes for up-regulation of
expression in response to this stimulus. The up-regulation in response to distention was
confirmed by quantitative Northern blot analysis both after a 4-hour in vitro distention and …
acute distention in the human fetal membranes. Study Design: Fetal membrane explants
were distended reproducibly in a novel device in vitro for 4 hours, and suppression
subtractive hybridization was used to identify the candidate genes for up-regulation of
expression in response to this stimulus. The up-regulation in response to distention was
confirmed by quantitative Northern blot analysis both after a 4-hour in vitro distention and …