Comparison of intrinsic activities of the putative sphingosine 1-phosphate receptor subtypes to regulate several signaling pathways in their cDNA-transfected Chinese …
J Kon, K Sato, T Watanabe, H Tomura… - Journal of Biological …, 1999 - ASBMB
We examined the actions of sphingosine 1-phosphate (S1P) on signaling pathways in
Chinese hamster ovary cells transfected with putative S1P receptor subtypes, ie Edg-1,
AGR16/H218 (Edg-5), and Edg-3. Among these receptor-transfected cells, there was no
significant difference in the expressing numbers of the S1P receptors and their affinities to
S1P, which were estimated by [3 H] S1P binding to the cells. In vector-transfected cells, S1P
slightly increased cytosolic Ca 2+ concentration ([Ca 2+] i) in association with inositol …
Chinese hamster ovary cells transfected with putative S1P receptor subtypes, ie Edg-1,
AGR16/H218 (Edg-5), and Edg-3. Among these receptor-transfected cells, there was no
significant difference in the expressing numbers of the S1P receptors and their affinities to
S1P, which were estimated by [3 H] S1P binding to the cells. In vector-transfected cells, S1P
slightly increased cytosolic Ca 2+ concentration ([Ca 2+] i) in association with inositol …
